Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies

Tissue samples from mosquitoes artificially infected with Zika virus and shown to be positive by RT-qPCR were reexamined by RT-PCR. Using these samples we compared the two methods employed in virus RNA detection for vector competence studies. Results demonstrated that, albeit useful, RT-PCR gave fal...

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Detalles Bibliográficos
Autores principales: Paiva, Marcelo Henrique Santos, Guedes, Duschinka Ribeiro Duarte, Leal, Walter Soares, Ayres, Constância Flávia Junqueira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Genética 2017
Materias:
Human and Medical Genetics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5596374/
https://www.ncbi.nlm.nih.gov/pubmed/28534930
http://dx.doi.org/10.1590/1678-4685-GMB-2016-0312

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5596374/
https://www.ncbi.nlm.nih.gov/pubmed/28534930
http://dx.doi.org/10.1590/1678-4685-GMB-2016-0312

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