Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies

Tissue samples from mosquitoes artificially infected with Zika virus and shown to be positive by RT-qPCR were reexamined by RT-PCR. Using these samples we compared the two methods employed in virus RNA detection for vector competence studies. Results demonstrated that, albeit useful, RT-PCR gave fal...

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Autores principales: Paiva, Marcelo Henrique Santos, Guedes, Duschinka Ribeiro Duarte, Leal, Walter Soares, Ayres, Constância Flávia Junqueira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Genética 2017
Materias:
Human and Medical Genetics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5596374/
https://www.ncbi.nlm.nih.gov/pubmed/28534930
http://dx.doi.org/10.1590/1678-4685-GMB-2016-0312
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author Paiva, Marcelo Henrique Santos
Guedes, Duschinka Ribeiro Duarte
Leal, Walter Soares
Ayres, Constância Flávia Junqueira
author_facet Paiva, Marcelo Henrique Santos
Guedes, Duschinka Ribeiro Duarte
Leal, Walter Soares
Ayres, Constância Flávia Junqueira
author_sort Paiva, Marcelo Henrique Santos
collection PubMed
description Tissue samples from mosquitoes artificially infected with Zika virus and shown to be positive by RT-qPCR were reexamined by RT-PCR. Using these samples we compared the two methods employed in virus RNA detection for vector competence studies. Results demonstrated that, albeit useful, RT-PCR gave false negatives with low viral loads (< 10(6) RNA copies/ml).
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spelling pubmed-55963742017-09-20 Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies Paiva, Marcelo Henrique Santos Guedes, Duschinka Ribeiro Duarte Leal, Walter Soares Ayres, Constância Flávia Junqueira Genet Mol Biol Human and Medical Genetics Tissue samples from mosquitoes artificially infected with Zika virus and shown to be positive by RT-qPCR were reexamined by RT-PCR. Using these samples we compared the two methods employed in virus RNA detection for vector competence studies. Results demonstrated that, albeit useful, RT-PCR gave false negatives with low viral loads (< 10(6) RNA copies/ml). Sociedade Brasileira de Genética 2017-05-22 2017 /pmc/articles/PMC5596374/ /pubmed/28534930 http://dx.doi.org/10.1590/1678-4685-GMB-2016-0312 Text en Copyright © 2017, Sociedade Brasileira de Genética. http://creativecommons.org/licenses/by/4.0/ License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License (type CC-BY), which permits unrestricted use, distribution and reproduction in any medium, provided the original article is properly cited.
spellingShingle Human and Medical Genetics
Paiva, Marcelo Henrique Santos
Guedes, Duschinka Ribeiro Duarte
Leal, Walter Soares
Ayres, Constância Flávia Junqueira
Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
title Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
title_full Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
title_fullStr Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
title_full_unstemmed Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
title_short Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
title_sort sensitivity of rt-pcr method in samples shown to be positive for zika virus by rt-qpcr in vector competence studies
topic Human and Medical Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5596374/
https://www.ncbi.nlm.nih.gov/pubmed/28534930
http://dx.doi.org/10.1590/1678-4685-GMB-2016-0312
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