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Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
Tissue samples from mosquitoes artificially infected with Zika virus and shown to be positive by RT-qPCR were reexamined by RT-PCR. Using these samples we compared the two methods employed in virus RNA detection for vector competence studies. Results demonstrated that, albeit useful, RT-PCR gave fal...
Autores principales: | Paiva, Marcelo Henrique Santos, Guedes, Duschinka Ribeiro Duarte, Leal, Walter Soares, Ayres, Constância Flávia Junqueira |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Genética
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5596374/ https://www.ncbi.nlm.nih.gov/pubmed/28534930 http://dx.doi.org/10.1590/1678-4685-GMB-2016-0312 |
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