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An Investigation into Spent Coffee Waste as a Renewable Source of Bioactive Compounds and Industrially Important Sugars

Conventional coffee brewing techniques generate vast quantities of spent espresso grounds (SEGs) rich in lignocellulose and valuable bioactives. These bioactive compounds can be exploited as a nutraceutical or used in a range of food products, while breakdown of lignocellulose generates metabolizabl...

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Autores principales: Scully, Damhan S., Jaiswal, Amit K., Abu-Ghannam, Nissreen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5597276/
https://www.ncbi.nlm.nih.gov/pubmed/28952594
http://dx.doi.org/10.3390/bioengineering3040033
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author Scully, Damhan S.
Jaiswal, Amit K.
Abu-Ghannam, Nissreen
author_facet Scully, Damhan S.
Jaiswal, Amit K.
Abu-Ghannam, Nissreen
author_sort Scully, Damhan S.
collection PubMed
description Conventional coffee brewing techniques generate vast quantities of spent espresso grounds (SEGs) rich in lignocellulose and valuable bioactives. These bioactive compounds can be exploited as a nutraceutical or used in a range of food products, while breakdown of lignocellulose generates metabolizable sugars that can be used for the production of various high-value products such as biofuels, amino acids and enzymes. Response surface methodology (RSM) was used to optimize the enzymatic saccharification of lignocellulose in SEGs following a hydrothermal pretreatment. A maximum reducing sugar yield was obtained at the following optimized hydrolysis conditions: 4.97 g of pretreated SEGs, 120 h reaction time, and 1246 and 250 µL of cellulase and hemicellulase, respectively. Industrially important sugars (glucose, galactose and mannose) were identified as the principal hydrolysis products under the studied conditions. Total flavonoids (p = 0.0002), total polyphenols (p = 0.03) and DPPH free-radical scavenging activity (p = 0.004) increased significantly after processing. A 14-fold increase in caffeine levels was also observed. This study provides insight into SEGs as a promising source of industrially important sugars and polyphenols.
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spelling pubmed-55972762017-09-21 An Investigation into Spent Coffee Waste as a Renewable Source of Bioactive Compounds and Industrially Important Sugars Scully, Damhan S. Jaiswal, Amit K. Abu-Ghannam, Nissreen Bioengineering (Basel) Article Conventional coffee brewing techniques generate vast quantities of spent espresso grounds (SEGs) rich in lignocellulose and valuable bioactives. These bioactive compounds can be exploited as a nutraceutical or used in a range of food products, while breakdown of lignocellulose generates metabolizable sugars that can be used for the production of various high-value products such as biofuels, amino acids and enzymes. Response surface methodology (RSM) was used to optimize the enzymatic saccharification of lignocellulose in SEGs following a hydrothermal pretreatment. A maximum reducing sugar yield was obtained at the following optimized hydrolysis conditions: 4.97 g of pretreated SEGs, 120 h reaction time, and 1246 and 250 µL of cellulase and hemicellulase, respectively. Industrially important sugars (glucose, galactose and mannose) were identified as the principal hydrolysis products under the studied conditions. Total flavonoids (p = 0.0002), total polyphenols (p = 0.03) and DPPH free-radical scavenging activity (p = 0.004) increased significantly after processing. A 14-fold increase in caffeine levels was also observed. This study provides insight into SEGs as a promising source of industrially important sugars and polyphenols. MDPI 2016-11-21 /pmc/articles/PMC5597276/ /pubmed/28952594 http://dx.doi.org/10.3390/bioengineering3040033 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Scully, Damhan S.
Jaiswal, Amit K.
Abu-Ghannam, Nissreen
An Investigation into Spent Coffee Waste as a Renewable Source of Bioactive Compounds and Industrially Important Sugars
title An Investigation into Spent Coffee Waste as a Renewable Source of Bioactive Compounds and Industrially Important Sugars
title_full An Investigation into Spent Coffee Waste as a Renewable Source of Bioactive Compounds and Industrially Important Sugars
title_fullStr An Investigation into Spent Coffee Waste as a Renewable Source of Bioactive Compounds and Industrially Important Sugars
title_full_unstemmed An Investigation into Spent Coffee Waste as a Renewable Source of Bioactive Compounds and Industrially Important Sugars
title_short An Investigation into Spent Coffee Waste as a Renewable Source of Bioactive Compounds and Industrially Important Sugars
title_sort investigation into spent coffee waste as a renewable source of bioactive compounds and industrially important sugars
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5597276/
https://www.ncbi.nlm.nih.gov/pubmed/28952594
http://dx.doi.org/10.3390/bioengineering3040033
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