Rapid Generation of Human Genetic Loss-of-Function iPSC Lines by Simultaneous Reprogramming and Gene Editing

Specifically ablating genes in human induced pluripotent stem cells (iPSCs) allows for studies of gene function as well as disease mechanisms in disorders caused by loss-of-function (LOF) mutations. While techniques exist for engineering such lines, we have developed and rigorously validated a metho...

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Detalles Bibliográficos
Autores principales: Tidball, Andrew M., Dang, Louis T., Glenn, Trevor W., Kilbane, Emma G., Klarr, Daniel J., Margolis, Joshua L., Uhler, Michael D., Parent, Jack M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5599229/
https://www.ncbi.nlm.nih.gov/pubmed/28781079
http://dx.doi.org/10.1016/j.stemcr.2017.07.003

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5599229/
https://www.ncbi.nlm.nih.gov/pubmed/28781079
http://dx.doi.org/10.1016/j.stemcr.2017.07.003

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