A novel SH2 recognition mechanism recruits Spt6 to the doubly phosphorylated RNA polymerase II linker at sites of transcription

We determined that the tandem SH2 domain of S. cerevisiae Spt6 binds the linker region of the RNA polymerase II subunit Rpb1 rather than the expected sites in its heptad repeat domain. The 4 nM binding affinity requires phosphorylation at Rpb1 S1493 and either T1471 or Y1473. Crystal structures show...

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Detalles Bibliográficos
Autores principales: Sdano, Matthew A, Fulcher, James M, Palani, Sowmiya, Chandrasekharan, Mahesh B, Parnell, Timothy J, Whitby, Frank G, Formosa, Tim, Hill, Christopher P
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2017
Materias:
Structural Biology and Molecular Biophysics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5599234/
https://www.ncbi.nlm.nih.gov/pubmed/28826505
http://dx.doi.org/10.7554/eLife.28723

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5599234/
https://www.ncbi.nlm.nih.gov/pubmed/28826505
http://dx.doi.org/10.7554/eLife.28723

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