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Profiling bacterial communities by MinION sequencing of ribosomal operons

BACKGROUND: An approach utilizing the long-read capability of the Oxford Nanopore MinION to rapidly sequence bacterial ribosomal operons of complex natural communities was developed. Microbial fingerprinting employs domain-specific forward primers (16S rRNA subunit), reverse primers (23S rRNA subuni...

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Detalles Bibliográficos
Autores principales: Kerkhof, Lee J., Dillon, Kevin P., Häggblom, Max M., McGuinness, Lora R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5599880/
https://www.ncbi.nlm.nih.gov/pubmed/28911333
http://dx.doi.org/10.1186/s40168-017-0336-9