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Isothermal titration calorimetry uncovers substrate promiscuity of bicupin oxalate oxidase from Ceriporiopsis subvermispora

Isothermal titration calorimetry (ITC) may be used to determine the kinetic parameters of enzyme-catalyzed reactions when neither products nor reactants are spectrophotometrically visible and when the reaction products are unknown. We report here the use of the multiple injection method of ITC to ch...

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Detalles Bibliográficos
Autores principales: Rana, Hassan, Moussatche, Patricia, Rocha, Lis Souza, Abdellaoui, Sofiene, Minteer, Shelley D., Moomaw, Ellen W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5600335/
https://www.ncbi.nlm.nih.gov/pubmed/28955847
http://dx.doi.org/10.1016/j.bbrep.2016.01.016