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Ribonucleoprotein purification and characterization using RNA Mango

The characterization of RNA–protein complexes (RNPs) is a difficult but increasingly important problem in modern biology. By combining the compact RNA Mango aptamer with a fluorogenic thiazole orange desthiobiotin (TO1-Dtb or TO3-Dtb) ligand, we have created an RNA tagging system that simplifies the...

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Detalles Bibliográficos
Autores principales: Panchapakesan, Shanker Shyam S., Ferguson, Matthew L., Hayden, Eric J., Chen, Xin, Hoskins, Aaron A., Unrau, Peter J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5602116/
https://www.ncbi.nlm.nih.gov/pubmed/28747322
http://dx.doi.org/10.1261/rna.062166.117