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Ribonucleoprotein purification and characterization using RNA Mango
The characterization of RNA–protein complexes (RNPs) is a difficult but increasingly important problem in modern biology. By combining the compact RNA Mango aptamer with a fluorogenic thiazole orange desthiobiotin (TO1-Dtb or TO3-Dtb) ligand, we have created an RNA tagging system that simplifies the...
Autores principales: | Panchapakesan, Shanker Shyam S., Ferguson, Matthew L., Hayden, Eric J., Chen, Xin, Hoskins, Aaron A., Unrau, Peter J. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5602116/ https://www.ncbi.nlm.nih.gov/pubmed/28747322 http://dx.doi.org/10.1261/rna.062166.117 |
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