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Construction of an HRP-streptavidin bound antigen and its application in an ELISA for porcine circovirus 2 antibodies

A fusion protein SBP-Cap∆41, consisting of Cap∆41 (without 41 amino acids at the N-terminus) protein of porcine circovirus 2 (PCV2) and a streptavidin binding peptide (SBP), was constructed. This fusion protein binds to HRP-labeled streptavidin (HRP-SA) through high affinity between SBP and SA, form...

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Detalles Bibliográficos
Autores principales: Ge, Meng, Li, Run-Cheng, Qu, Tailong, Gong, Wenjie, Yu, Xing-Long, Tu, Changchun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5603472/
https://www.ncbi.nlm.nih.gov/pubmed/28921455
http://dx.doi.org/10.1186/s13568-017-0473-3