Single copy-sensitive electrochemical assay for circulating methylated DNA in clinical samples with ultrahigh specificity based on a sequential discrimination–amplification strategy

Tumor-related circulating methylated DNA represents only a small fraction of the total DNA in clinical samples (e.g. plasma), challenging the accurate analysis of specific DNA methylation patterns. Yet conventional assays based on the real-time quantitative methylation-specific PCR (qMSP) are genera...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Xuyao, Chen, Feng, Zhang, Dexin, Zhao, Yue, Wei, Jing, Wang, Lihua, Song, Shiping, Fan, Chunhai, Zhao, Yongxi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2017
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5603958/
https://www.ncbi.nlm.nih.gov/pubmed/28959399
http://dx.doi.org/10.1039/c7sc01035d

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5603958/
https://www.ncbi.nlm.nih.gov/pubmed/28959399
http://dx.doi.org/10.1039/c7sc01035d

Ejemplares similares