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Development and evaluation of an efficient heterologous gene knock-in reporter system in Lactococcus lactis
BACKGROUND: Lactococcus lactis is a food grade probiotics and widely used to express heterologous proteins. Generally, target genes are knocked into the L. lactis genome through double-crossover recombination to express heterologous proteins stably. However, creating marker-less heterologous genes k...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5604289/ https://www.ncbi.nlm.nih.gov/pubmed/28923077 http://dx.doi.org/10.1186/s12934-017-0770-1 |