Development and evaluation of an efficient heterologous gene knock-in reporter system in Lactococcus lactis

BACKGROUND: Lactococcus lactis is a food grade probiotics and widely used to express heterologous proteins. Generally, target genes are knocked into the L. lactis genome through double-crossover recombination to express heterologous proteins stably. However, creating marker-less heterologous genes k...

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Detalles Bibliográficos
Autores principales: Lu, Yifei, Yan, Hongxiang, Deng, Jiezhong, Huang, Zhigang, Jin, Xurui, Yu, Yanlan, Hu, Qiwen, Hu, Fuquan, Wang, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5604289/
https://www.ncbi.nlm.nih.gov/pubmed/28923077
http://dx.doi.org/10.1186/s12934-017-0770-1

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