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Automatic and adaptive heterogeneous refractive index compensation for light-sheet microscopy
Optical tissue clearing has revolutionized researchers’ ability to perform fluorescent measurements of molecules, cells, and structures within intact tissue. One common complication to all optically cleared tissue is a spatially heterogeneous refractive index, leading to light scattering and first-o...
Autores principales: | Ryan, Duncan P., Gould, Elizabeth A., Seedorf, Gregory J., Masihzadeh, Omid, Abman, Steven H., Vijayaraghavan, Sukumar, Macklin, Wendy B., Restrepo, Diego, Shepherd, Douglas P. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5606987/ https://www.ncbi.nlm.nih.gov/pubmed/28931809 http://dx.doi.org/10.1038/s41467-017-00514-7 |
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