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Single molecule counting and assessment of random molecular tagging errors with transposable giga-scale error-correcting barcodes

BACKGROUND: RNA-Seq measures gene expression by counting sequence reads belonging to unique cDNA fragments. Molecular barcodes commonly in the form of random nucleotides were recently introduced to improve gene expression measures by detecting amplification duplicates, but are susceptible to errors...

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Detalles Bibliográficos
Autores principales: Lau, Billy T., Ji, Hanlee P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5609065/
https://www.ncbi.nlm.nih.gov/pubmed/28934929
http://dx.doi.org/10.1186/s12864-017-4141-4