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Single molecule counting and assessment of random molecular tagging errors with transposable giga-scale error-correcting barcodes
BACKGROUND: RNA-Seq measures gene expression by counting sequence reads belonging to unique cDNA fragments. Molecular barcodes commonly in the form of random nucleotides were recently introduced to improve gene expression measures by detecting amplification duplicates, but are susceptible to errors...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5609065/ https://www.ncbi.nlm.nih.gov/pubmed/28934929 http://dx.doi.org/10.1186/s12864-017-4141-4 |