Efficient generation of mutations mediated by CRISPR/Cas9 in the hairy root transformation system of Brassica carinata

A protocol for the induction of site-directed deletions and insertions in the genome of Brassica carinata with CRISPR is described. The construct containing the Cas9 nuclease and the guide RNA (gRNA) was delivered by the hairy root transformation technique, and a successful transformation was monito...

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Detalles Bibliográficos
Autores principales: Kirchner, Thomas W., Niehaus, Markus, Debener, Thomas, Schenk, Manfred K., Herde, Marco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5609758/
https://www.ncbi.nlm.nih.gov/pubmed/28937992
http://dx.doi.org/10.1371/journal.pone.0185429

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5609758/
https://www.ncbi.nlm.nih.gov/pubmed/28937992
http://dx.doi.org/10.1371/journal.pone.0185429

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