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Efficient generation of mutations mediated by CRISPR/Cas9 in the hairy root transformation system of Brassica carinata
A protocol for the induction of site-directed deletions and insertions in the genome of Brassica carinata with CRISPR is described. The construct containing the Cas9 nuclease and the guide RNA (gRNA) was delivered by the hairy root transformation technique, and a successful transformation was monito...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5609758/ https://www.ncbi.nlm.nih.gov/pubmed/28937992 http://dx.doi.org/10.1371/journal.pone.0185429 |