A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer

CRISPR/Cas9 technology has greatly improved the feasibility and speed of loss-of-function studies that are essential in understanding gene function. In higher eukaryotes, paralogous genes can mask a potential phenotype by compensating the loss of a gene, thus limiting the information that can be obt...

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Detalles Bibliográficos
Autores principales: Merenda, Alessandra, Andersson-Rolf, Amanda, Mustata, Roxana C., Li, Taibo, Kim, Hyunki, Koo, Bon-Kyoung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2017
Materias:
Bioengineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5612278/
https://www.ncbi.nlm.nih.gov/pubmed/28745625
http://dx.doi.org/10.3791/55916

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5612278/
https://www.ncbi.nlm.nih.gov/pubmed/28745625
http://dx.doi.org/10.3791/55916

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