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Application of a novel HiBiT peptide tag for monitoring ATF4 protein expression in Neuro2a cells

A split NanoLuc assay system consisting of two fragments, large N-terminal and small C-terminal regions (NanoBiT), was developed to investigate protein-protein interactions within living cells. Interestingly, the replacement of five amino acids among 11 C-terminal amino acids dramatically increased...

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Detalles Bibliográficos
Autores principales: Oh-hashi, Kentaro, Furuta, Eri, Fujimura, Keito, Hirata, Yoko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5613219/
https://www.ncbi.nlm.nih.gov/pubmed/28955790
http://dx.doi.org/10.1016/j.bbrep.2017.08.002