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Post-pulse addition of trans-cyclohexane-1,2-diol improves electrotransfer mediated gene expression in mammalian cells

Electric field mediated gene transfer is facing a problem in expression yield due to the poor transfer across the nuclear envelope. Trans-cyclohexane-1,2-diol (TCHD) was shown to significantly increase chemically mediated transfection by collapsing the permeability barrier of the nuclear pore comple...

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Detalles Bibliográficos
Autores principales: Pasquet, L., Bellard, E., Rols, M.P., Golzio, M., Teissie, J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5613639/
https://www.ncbi.nlm.nih.gov/pubmed/28955917
http://dx.doi.org/10.1016/j.bbrep.2016.07.012