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Mechanistic investigation of capability of enzymatically synthesized polycysteine to cross-link proteins
BACKGROUND: Previously, we had reported that α-chymotrypsin–catalyzed polymerization of l-cysteine ethyl ester in a frozen buffer provided poly-l-cysteine (PLCys) in good yield, of which degree of polymerization had been determined to be 6–11. Almost all of SH groups in PLCys were in free forms. Suc...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2016
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5613652/ https://www.ncbi.nlm.nih.gov/pubmed/28955924 http://dx.doi.org/10.1016/j.bbrep.2016.07.013 |