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Purification and characterization of trypsin from Luphiosilurus alexandri pyloric cecum

Trypsin from L. alexandri was purified using only two purification processes: ammonium sulfate precipitation and anion exchange liquid chromatography in DEAE-Sepharose. Trypsin mass was estimated as 24 kDa through SDS-PAGE, which showed only one band in silver staining. The purified enzyme showed an...

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Detalles Bibliográficos
Autores principales: dos Santos, Claudio Wilian Victor, da Costa Marques, Maria Elizabeth, de Araújo Tenório, Humberto, de Miranda, Edma Carvalho, Vieira Pereira, Hugo Juarez
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5613698/
https://www.ncbi.nlm.nih.gov/pubmed/28955938
http://dx.doi.org/10.1016/j.bbrep.2016.08.003