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Purification and characterization of trypsin from Luphiosilurus alexandri pyloric cecum
Trypsin from L. alexandri was purified using only two purification processes: ammonium sulfate precipitation and anion exchange liquid chromatography in DEAE-Sepharose. Trypsin mass was estimated as 24 kDa through SDS-PAGE, which showed only one band in silver staining. The purified enzyme showed an...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5613698/ https://www.ncbi.nlm.nih.gov/pubmed/28955938 http://dx.doi.org/10.1016/j.bbrep.2016.08.003 |