Efficient DNA ligation by selective heating of DNA ligase with a radio frequency alternating magnetic field

We present a simple method for efficient DNA ligation utilizing the heat generation of ferromagnetic particles subjected to an ac magnetic field. We carry out the ligation of DNA fragments with cohesive ends using T4 DNA ligase immobilized on the surface of ferromagnetic particles. When a radio freq...

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Detalles Bibliográficos
Autores principales: Suzuki, Masashi, Hayashi, Hiroaki, Mizuki, Toru, Maekawa, Toru, Morimoto, Hisao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5614472/
https://www.ncbi.nlm.nih.gov/pubmed/28955977
http://dx.doi.org/10.1016/j.bbrep.2016.10.006

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5614472/
https://www.ncbi.nlm.nih.gov/pubmed/28955977
http://dx.doi.org/10.1016/j.bbrep.2016.10.006

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