Evaluation of the efficiency and utility of recombinant enzyme-free seamless DNA cloning methods

Simple and low-cost recombinant enzyme-free seamless DNA cloning methods have recently become available. In vivo Escherichia coli cloning (iVEC) can directly transform a mixture of insert and vector DNA fragments into E. coli, which are ligated by endogenous homologous recombination activity in the...

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Detalles Bibliográficos
Autor principal: Motohashi, Ken
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5614619/
https://www.ncbi.nlm.nih.gov/pubmed/28956018
http://dx.doi.org/10.1016/j.bbrep.2017.01.010

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5614619/
https://www.ncbi.nlm.nih.gov/pubmed/28956018
http://dx.doi.org/10.1016/j.bbrep.2017.01.010

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