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Evaluation of the efficiency and utility of recombinant enzyme-free seamless DNA cloning methods
Simple and low-cost recombinant enzyme-free seamless DNA cloning methods have recently become available. In vivo Escherichia coli cloning (iVEC) can directly transform a mixture of insert and vector DNA fragments into E. coli, which are ligated by endogenous homologous recombination activity in the...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Elsevier
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5614619/ https://www.ncbi.nlm.nih.gov/pubmed/28956018 http://dx.doi.org/10.1016/j.bbrep.2017.01.010 |