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Quantification of Pseudouridine Levels in Cellular RNA Pools with a Modified HPLC-UV Assay

Pseudouridine (Ψ) is the most abundant post-transcriptionally modified ribonucleoside. Different Ψ modifications correlate with stress responses and are postulated to coordinate the distinct biological responses to a diverse panel of cellular stresses. With the help of different guide RNAs, the dysk...

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Detalles Bibliográficos
Autores principales: Xu, Jialin, Gu, Alice Y., Thumati, Naresh R., Wong, Judy M.Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5615352/
https://www.ncbi.nlm.nih.gov/pubmed/28872587
http://dx.doi.org/10.3390/genes8090219