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Development of a Rapid Method for the Determination of Caffeine in Coffee Grains by GC-FID—A Fully Validated Approach
A simple method for the determination of caffeine in coffee grains by GC-FID (Gas Chromatography-Flame Ionisation Detector) is presented in the current work. The method was fully validated according to ISO (International Organization for Standardization) 17025 requirements and European Commission re...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618095/ https://www.ncbi.nlm.nih.gov/pubmed/28829349 http://dx.doi.org/10.3390/antiox6030067 |
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author | Pasias, Ioannis N. Kiriakou, I. Proestos, Charalampos |
author_facet | Pasias, Ioannis N. Kiriakou, I. Proestos, Charalampos |
author_sort | Pasias, Ioannis N. |
collection | PubMed |
description | A simple method for the determination of caffeine in coffee grains by GC-FID (Gas Chromatography-Flame Ionisation Detector) is presented in the current work. The method was fully validated according to ISO (International Organization for Standardization) 17025 requirements and European Commission regulations. The accuracy, as provided by recovery experiments, was higher than 93%, and the precision, as provided by the (%) relative standard deviation under reproducibility conditions, was lower than 5%. A vast number of independent parameters that lead in the increase of uncertainty of methods were investigated. The analysis was performed without use of an internal standard, which was proven to be reliable according to several validation methods. The method was applied in real samples, and possible health claims were investigated. |
format | Online Article Text |
id | pubmed-5618095 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-56180952017-09-29 Development of a Rapid Method for the Determination of Caffeine in Coffee Grains by GC-FID—A Fully Validated Approach Pasias, Ioannis N. Kiriakou, I. Proestos, Charalampos Antioxidants (Basel) Article A simple method for the determination of caffeine in coffee grains by GC-FID (Gas Chromatography-Flame Ionisation Detector) is presented in the current work. The method was fully validated according to ISO (International Organization for Standardization) 17025 requirements and European Commission regulations. The accuracy, as provided by recovery experiments, was higher than 93%, and the precision, as provided by the (%) relative standard deviation under reproducibility conditions, was lower than 5%. A vast number of independent parameters that lead in the increase of uncertainty of methods were investigated. The analysis was performed without use of an internal standard, which was proven to be reliable according to several validation methods. The method was applied in real samples, and possible health claims were investigated. MDPI 2017-08-22 /pmc/articles/PMC5618095/ /pubmed/28829349 http://dx.doi.org/10.3390/antiox6030067 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pasias, Ioannis N. Kiriakou, I. Proestos, Charalampos Development of a Rapid Method for the Determination of Caffeine in Coffee Grains by GC-FID—A Fully Validated Approach |
title | Development of a Rapid Method for the Determination of Caffeine in Coffee Grains by GC-FID—A Fully Validated Approach |
title_full | Development of a Rapid Method for the Determination of Caffeine in Coffee Grains by GC-FID—A Fully Validated Approach |
title_fullStr | Development of a Rapid Method for the Determination of Caffeine in Coffee Grains by GC-FID—A Fully Validated Approach |
title_full_unstemmed | Development of a Rapid Method for the Determination of Caffeine in Coffee Grains by GC-FID—A Fully Validated Approach |
title_short | Development of a Rapid Method for the Determination of Caffeine in Coffee Grains by GC-FID—A Fully Validated Approach |
title_sort | development of a rapid method for the determination of caffeine in coffee grains by gc-fid—a fully validated approach |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618095/ https://www.ncbi.nlm.nih.gov/pubmed/28829349 http://dx.doi.org/10.3390/antiox6030067 |
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