Controlling Protein Surface Orientation by Strategic Placement of Oligo-Histidine Tags

[Image: see text] We report oriented immobilization of proteins using the standard hexahistidine (His(6))-Ni(2+):NTA (nitrilotriacetic acid) methodology, which we systematically tuned to give control of surface coverage. Fluorescence microscopy and surface plasmon resonance measurements of self-asse...

Descripción completa

Detalles Bibliográficos
Autores principales: Wasserberg, Dorothee, Cabanas-Danés, Jordi, Prangsma, Jord, O’Mahony, Shane, Cazade, Pierre-Andre, Tromp, Eldrich, Blum, Christian, Thompson, Damien, Huskens, Jurriaan, Subramaniam, Vinod, Jonkheijm, Pascal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2017
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618149/
https://www.ncbi.nlm.nih.gov/pubmed/28850777
http://dx.doi.org/10.1021/acsnano.7b03717

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618149/
https://www.ncbi.nlm.nih.gov/pubmed/28850777
http://dx.doi.org/10.1021/acsnano.7b03717

Ejemplares similares