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Controlling Protein Surface Orientation by Strategic Placement of Oligo-Histidine Tags

[Image: see text] We report oriented immobilization of proteins using the standard hexahistidine (His(6))-Ni(2+):NTA (nitrilotriacetic acid) methodology, which we systematically tuned to give control of surface coverage. Fluorescence microscopy and surface plasmon resonance measurements of self-asse...

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Detalles Bibliográficos
Autores principales:	Wasserberg, Dorothee, Cabanas-Danés, Jordi, Prangsma, Jord, O’Mahony, Shane, Cazade, Pierre-Andre, Tromp, Eldrich, Blum, Christian, Thompson, Damien, Huskens, Jurriaan, Subramaniam, Vinod, Jonkheijm, Pascal
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2017
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618149/ https://www.ncbi.nlm.nih.gov/pubmed/28850777 http://dx.doi.org/10.1021/acsnano.7b03717

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