Expression of short hairpin RNAs using the compact architecture of retroviral microRNA genes

Short hairpin RNAs (shRNAs) are effective in generating stable repression of gene expression. RNA polymerase III (RNAP III) type III promoters (U6 or H1) are typically used to drive shRNA expression. While useful for some knockdown applications, the robust expression of U6/H1-driven shRNAs can induc...

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Detalles Bibliográficos
Autores principales: Burke, James M., Kincaid, Rodney P., Aloisio, Francesca, Welch, Nicole, Sullivan, Christopher S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5622367/
https://www.ncbi.nlm.nih.gov/pubmed/28973449
http://dx.doi.org/10.1093/nar/gkx653

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5622367/
https://www.ncbi.nlm.nih.gov/pubmed/28973449
http://dx.doi.org/10.1093/nar/gkx653

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