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Identification of simvastatin-regulated targets associated with JNK activation in DU145 human prostate cancer cell death signaling
The results of this study show that c-Jun N-terminal kinase (JNK) activation was associated with the enhancement of docetaxel-induced cytotoxicity by simvastatin in DU145 human prostate cancer cells. To better understand the basic molecular mechanisms, we investigated simvastatin-regulated targets d...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society for Biochemistry and Molecular Biology
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5625694/ https://www.ncbi.nlm.nih.gov/pubmed/28803608 http://dx.doi.org/10.5483/BMBRep.2017.50.9.087 |
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author | Jung, Eun Joo Chung, Ky Hyun Kim, Choong Won |
author_facet | Jung, Eun Joo Chung, Ky Hyun Kim, Choong Won |
author_sort | Jung, Eun Joo |
collection | PubMed |
description | The results of this study show that c-Jun N-terminal kinase (JNK) activation was associated with the enhancement of docetaxel-induced cytotoxicity by simvastatin in DU145 human prostate cancer cells. To better understand the basic molecular mechanisms, we investigated simvastatin-regulated targets during simvastatin-induced cell death in DU145 cells using two-dimensional (2D) proteomic analysis. Thus, vimentin, Ras-related protein Rab-1B (RAB1B), cytoplasmic hydroxymethylglutaryl-CoA synthase (cHMGCS), thioredoxin domain-containing protein 5 (TXNDC5), heterogeneous nuclear ribonucleoprotein K (hnRNP K), N-myc downstream-regulated gene 1 (NDRG1), and isopentenyl-diphosphate Delta-isomerase 1 (IDI1) protein spots were identified as simvastatin-regulated targets involved in DU145 cell death signaling pathways. Moreover, the JNK inhibitor SP600125 significantly inhibited the upregulation of NDRG1 and IDI protein levels by combination treatment of docetaxel and simvastatin. These results suggest that NDRG1 and IDI could at least play an important role in DU145 cell death signaling as simvastatin-regulated targets associated with JNK activation. |
format | Online Article Text |
id | pubmed-5625694 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Korean Society for Biochemistry and Molecular Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-56256942017-10-10 Identification of simvastatin-regulated targets associated with JNK activation in DU145 human prostate cancer cell death signaling Jung, Eun Joo Chung, Ky Hyun Kim, Choong Won BMB Rep Articles The results of this study show that c-Jun N-terminal kinase (JNK) activation was associated with the enhancement of docetaxel-induced cytotoxicity by simvastatin in DU145 human prostate cancer cells. To better understand the basic molecular mechanisms, we investigated simvastatin-regulated targets during simvastatin-induced cell death in DU145 cells using two-dimensional (2D) proteomic analysis. Thus, vimentin, Ras-related protein Rab-1B (RAB1B), cytoplasmic hydroxymethylglutaryl-CoA synthase (cHMGCS), thioredoxin domain-containing protein 5 (TXNDC5), heterogeneous nuclear ribonucleoprotein K (hnRNP K), N-myc downstream-regulated gene 1 (NDRG1), and isopentenyl-diphosphate Delta-isomerase 1 (IDI1) protein spots were identified as simvastatin-regulated targets involved in DU145 cell death signaling pathways. Moreover, the JNK inhibitor SP600125 significantly inhibited the upregulation of NDRG1 and IDI protein levels by combination treatment of docetaxel and simvastatin. These results suggest that NDRG1 and IDI could at least play an important role in DU145 cell death signaling as simvastatin-regulated targets associated with JNK activation. Korean Society for Biochemistry and Molecular Biology 2017-09 2017-09-30 /pmc/articles/PMC5625694/ /pubmed/28803608 http://dx.doi.org/10.5483/BMBRep.2017.50.9.087 Text en Copyright © 2017 by the The Korean Society for Biochemistry and Molecular Biology http://creativecommons.org/licenses/by-nc/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Jung, Eun Joo Chung, Ky Hyun Kim, Choong Won Identification of simvastatin-regulated targets associated with JNK activation in DU145 human prostate cancer cell death signaling |
title | Identification of simvastatin-regulated targets associated with JNK activation in DU145 human prostate cancer cell death signaling |
title_full | Identification of simvastatin-regulated targets associated with JNK activation in DU145 human prostate cancer cell death signaling |
title_fullStr | Identification of simvastatin-regulated targets associated with JNK activation in DU145 human prostate cancer cell death signaling |
title_full_unstemmed | Identification of simvastatin-regulated targets associated with JNK activation in DU145 human prostate cancer cell death signaling |
title_short | Identification of simvastatin-regulated targets associated with JNK activation in DU145 human prostate cancer cell death signaling |
title_sort | identification of simvastatin-regulated targets associated with jnk activation in du145 human prostate cancer cell death signaling |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5625694/ https://www.ncbi.nlm.nih.gov/pubmed/28803608 http://dx.doi.org/10.5483/BMBRep.2017.50.9.087 |
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