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Microsecond time-scale kinetics of transient biochemical reactions

To afford mechanistic studies in enzyme kinetics and protein folding in the microsecond time domain we have developed a continuous-flow microsecond time-scale mixing instrument with an unprecedented dead-time of 3.8 ± 0.3 μs. The instrument employs a micro-mixer with a mixing time of 2.7 μs integrat...

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Detalles Bibliográficos
Autores principales: Mitić, Sandra, Strampraad, Marc J. F., Hagen, Wilfred R., de Vries, Simon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5626514/
https://www.ncbi.nlm.nih.gov/pubmed/28973014
http://dx.doi.org/10.1371/journal.pone.0185888