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Microsecond time-scale kinetics of transient biochemical reactions
To afford mechanistic studies in enzyme kinetics and protein folding in the microsecond time domain we have developed a continuous-flow microsecond time-scale mixing instrument with an unprecedented dead-time of 3.8 ± 0.3 μs. The instrument employs a micro-mixer with a mixing time of 2.7 μs integrat...
Autores principales: | Mitić, Sandra, Strampraad, Marc J. F., Hagen, Wilfred R., de Vries, Simon |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5626514/ https://www.ncbi.nlm.nih.gov/pubmed/28973014 http://dx.doi.org/10.1371/journal.pone.0185888 |
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