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In vitro synergistic activities of cefazolin and nisin A against mastitis pathogens

First-generation cephalosporins such as cefazolin (CEZ) have been widely used for mastitis treatment in dairy cattle. However, the use of antibiotics results in the presence of antibiotic residues in milk, which is used for human consumption. Nisin A, a bacteriocin produced by Lactococcus lactis, ha...

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Autores principales: KITAZAKI, Kohei, KOGA, Shoko, NAGATOSHI, Kohei, KUWANO, Koichi, ZENDO, Takeshi, NAKAYAMA, Jiro, SONOMOTO, Kenji, ANO, Hitoshi, KATAMOTO, Hiromu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5627315/
https://www.ncbi.nlm.nih.gov/pubmed/28757508
http://dx.doi.org/10.1292/jvms.17-0180
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author KITAZAKI, Kohei
KOGA, Shoko
NAGATOSHI, Kohei
KUWANO, Koichi
ZENDO, Takeshi
NAKAYAMA, Jiro
SONOMOTO, Kenji
ANO, Hitoshi
KATAMOTO, Hiromu
author_facet KITAZAKI, Kohei
KOGA, Shoko
NAGATOSHI, Kohei
KUWANO, Koichi
ZENDO, Takeshi
NAKAYAMA, Jiro
SONOMOTO, Kenji
ANO, Hitoshi
KATAMOTO, Hiromu
author_sort KITAZAKI, Kohei
collection PubMed
description First-generation cephalosporins such as cefazolin (CEZ) have been widely used for mastitis treatment in dairy cattle. However, the use of antibiotics results in the presence of antibiotic residues in milk, which is used for human consumption. Nisin A, a bacteriocin produced by Lactococcus lactis, has been used as a broad-spectrum food preservative for over 50 years. Therefore, a combination of CEZ and nisin A might provide an extended activity spectrum against mastitis pathogens and reduce the antibiotic dose for mastitis treatment. This study aimed to evaluate the combined effect of CEZ and nisin A against mastitis pathogens using the checkerboard and time-kill assays. In the checkerboard assay, the CEZ-nisin A combination exhibited a synergistic effect against Staphylococcus aureus (n=20/20) and Enterococcus faecalis (n=13/18), and meanwhile exhibited a mostly additive effect against Staphylococcus intermedius (n=12/20), Streptococcus agalactiae (n=10/10), Streptococcus dysgalactiae (n=18/18), and Escherichia coli (n=14/18). There were no indifferent or antagonistic effects between CEZ and nisin A. In the time-kill assay, the CEZ-nisin A combination at 0.5 × or 1 × minimum inhibitory concentration exhibited synergistic reduction of bacterial growth by over 3 log(10) colony forming units per ml relative to that observed with either antimicrobial substance alone. These results suggest that the CEZ-nisin A combination can be used for developing an intramammary infusion for mastitis treatment, with lower antibiotic concentrations than normal.
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spelling pubmed-56273152017-10-10 In vitro synergistic activities of cefazolin and nisin A against mastitis pathogens KITAZAKI, Kohei KOGA, Shoko NAGATOSHI, Kohei KUWANO, Koichi ZENDO, Takeshi NAKAYAMA, Jiro SONOMOTO, Kenji ANO, Hitoshi KATAMOTO, Hiromu J Vet Med Sci Bacteriology First-generation cephalosporins such as cefazolin (CEZ) have been widely used for mastitis treatment in dairy cattle. However, the use of antibiotics results in the presence of antibiotic residues in milk, which is used for human consumption. Nisin A, a bacteriocin produced by Lactococcus lactis, has been used as a broad-spectrum food preservative for over 50 years. Therefore, a combination of CEZ and nisin A might provide an extended activity spectrum against mastitis pathogens and reduce the antibiotic dose for mastitis treatment. This study aimed to evaluate the combined effect of CEZ and nisin A against mastitis pathogens using the checkerboard and time-kill assays. In the checkerboard assay, the CEZ-nisin A combination exhibited a synergistic effect against Staphylococcus aureus (n=20/20) and Enterococcus faecalis (n=13/18), and meanwhile exhibited a mostly additive effect against Staphylococcus intermedius (n=12/20), Streptococcus agalactiae (n=10/10), Streptococcus dysgalactiae (n=18/18), and Escherichia coli (n=14/18). There were no indifferent or antagonistic effects between CEZ and nisin A. In the time-kill assay, the CEZ-nisin A combination at 0.5 × or 1 × minimum inhibitory concentration exhibited synergistic reduction of bacterial growth by over 3 log(10) colony forming units per ml relative to that observed with either antimicrobial substance alone. These results suggest that the CEZ-nisin A combination can be used for developing an intramammary infusion for mastitis treatment, with lower antibiotic concentrations than normal. The Japanese Society of Veterinary Science 2017-07-29 2017-09 /pmc/articles/PMC5627315/ /pubmed/28757508 http://dx.doi.org/10.1292/jvms.17-0180 Text en ©2017 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Bacteriology
KITAZAKI, Kohei
KOGA, Shoko
NAGATOSHI, Kohei
KUWANO, Koichi
ZENDO, Takeshi
NAKAYAMA, Jiro
SONOMOTO, Kenji
ANO, Hitoshi
KATAMOTO, Hiromu
In vitro synergistic activities of cefazolin and nisin A against mastitis pathogens
title In vitro synergistic activities of cefazolin and nisin A against mastitis pathogens
title_full In vitro synergistic activities of cefazolin and nisin A against mastitis pathogens
title_fullStr In vitro synergistic activities of cefazolin and nisin A against mastitis pathogens
title_full_unstemmed In vitro synergistic activities of cefazolin and nisin A against mastitis pathogens
title_short In vitro synergistic activities of cefazolin and nisin A against mastitis pathogens
title_sort in vitro synergistic activities of cefazolin and nisin a against mastitis pathogens
topic Bacteriology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5627315/
https://www.ncbi.nlm.nih.gov/pubmed/28757508
http://dx.doi.org/10.1292/jvms.17-0180
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