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Integrating nested PCR with high-throughput sequencing to characterize mutations of HBV genome in low viral load samples
Due to the low viral load of hepatitis B virus (HBV) in plasma samples, conventional techniques have limitations to the detection of antiviral resistance mutations. To solve the problem, we developed a fast, highly sensitive, and accurate method to sequence the HBV whole-genome sequencing in plasma...
Autores principales: | Wang, Xianjun, Xu, Lihui, Chen, Yueming, Liu, Anbing, Wang, Liqian, Xu, Peisong, Liu, Yunhui, Li, Lei, Meng, Fei |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer Health
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5627833/ https://www.ncbi.nlm.nih.gov/pubmed/28746207 http://dx.doi.org/10.1097/MD.0000000000007588 |
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