Genomic GC-Content Affects the Accuracy of 16S rRNA Gene Sequencing Based Microbial Profiling due to PCR Bias

Profiling of microbial community composition is frequently performed by partial 16S rRNA gene sequencing on benchtop platforms following PCR amplification of specific hypervariable regions within this gene. Accuracy and reproducibility of this strategy are two key parameters to consider, which may b...

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Detalles Bibliográficos
Autores principales: Laursen, Martin F., Dalgaard, Marlene D., Bahl, Martin I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5633598/
https://www.ncbi.nlm.nih.gov/pubmed/29051756
http://dx.doi.org/10.3389/fmicb.2017.01934

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5633598/
https://www.ncbi.nlm.nih.gov/pubmed/29051756
http://dx.doi.org/10.3389/fmicb.2017.01934

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