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Introducing site-specific cysteines into nanobodies for mercury labelling allows de novo phasing of their crystal structures
The generation of high-quality protein crystals and the loss of phase information during an X-ray crystallography diffraction experiment represent the major bottlenecks in the determination of novel protein structures. A generic method for introducing Hg atoms into any crystal independent of the pre...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
International Union of Crystallography
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5633906/ https://www.ncbi.nlm.nih.gov/pubmed/28994409 http://dx.doi.org/10.1107/S2059798317013171 |