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Expression and Characterization of a Recombinant Endoglucanase From Western Corn Rootworm, in Pichia pastoris
The endoglucanase cDNA, Dvv-ENGase I, from western corn rootworm, Diabrotica virgifera virgifera LeConte was expressed using the GS115 methylotrophic strain of Pichia pastoris . The Dvv-ENGase I gene was cloned into the integrative plasmid pPICZαA under the control of AOX1, which is a methanol-induc...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5633935/ https://www.ncbi.nlm.nih.gov/pubmed/25434035 http://dx.doi.org/10.1093/jisesa/ieu104 |