Tolerance analysis of chloroplast OsCu/Zn-SOD overexpressing rice under NaCl and NaHCO(3) stress

The 636-bp-long cDNA sequence of OsCu/Zn-SOD (AK059841) was cloned from Oryza sativa var. Longjing11 via reverse transcription polymerase chain reaction (RT-PCR). The encoded protein comprised of 211 amino acids is highly homologous to Cu/Zn-SOD proteins from tuscacera rice and millet. Quantitative...

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Autores principales: Guan, Qingjie, Liao, Xu, He, Mingliang, Li, Xiufeng, Wang, Zhenyu, Ma, Haiyan, Yu, Song, Liu, Shenkui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5636109/
https://www.ncbi.nlm.nih.gov/pubmed/29020034
http://dx.doi.org/10.1371/journal.pone.0186052
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author Guan, Qingjie
Liao, Xu
He, Mingliang
Li, Xiufeng
Wang, Zhenyu
Ma, Haiyan
Yu, Song
Liu, Shenkui
author_facet Guan, Qingjie
Liao, Xu
He, Mingliang
Li, Xiufeng
Wang, Zhenyu
Ma, Haiyan
Yu, Song
Liu, Shenkui
author_sort Guan, Qingjie
collection PubMed
description The 636-bp-long cDNA sequence of OsCu/Zn-SOD (AK059841) was cloned from Oryza sativa var. Longjing11 via reverse transcription polymerase chain reaction (RT-PCR). The encoded protein comprised of 211 amino acids is highly homologous to Cu/Zn-SOD proteins from tuscacera rice and millet. Quantitative RT-PCR revealed that in rice, the level of OsCu/Zn-SOD gene expression was lowest in roots and was highest in petals and during the S5 leaf stage. Moreover, the expression level of OsCu/Zn-SOD gene expression decreased during the L5 leaf stage to maturity. The level of OsCu/Zn-SOD gene expression, however, was increased under saline–sodic stress and NaHCO(3) stress. Germination tests under 125, 150, and 175 mM NaCl revealed that OsCu/Zn-SOD-overexpressing lines performed better than the non-transgenic (NT) Longjing11 lines in terms of germination rate and height. Subjecting seedlings to NaHCO(3) and water stress revealed that OsCu/Zn-SOD-overexpressing lines performed better than NT in terms of SOD activity, fresh weight, root length, and height. Under simulated NaHCO(3) stress, OsCu/Zn-SOD-overexpressing lines performed better than NT in terms of survival rate (25.19% > 6.67%) and yield traits (average grain weight 20.6 > 18.15 g). This study showed that OsCu/Zn-SOD gene overexpression increases the detoxification capacity of reactive oxygen species in O. sativa and reduces salt-induced oxidative damage. We also revealed the regulatory mechanism of OsCu/Zn-SOD enzyme in saline–sodic stress resistance in O. sativa. Moreover, we provided an experimental foundation for studying the mechanism of OsCu/Zn-SOD enzymes in the chloroplast.
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spelling pubmed-56361092017-10-30 Tolerance analysis of chloroplast OsCu/Zn-SOD overexpressing rice under NaCl and NaHCO(3) stress Guan, Qingjie Liao, Xu He, Mingliang Li, Xiufeng Wang, Zhenyu Ma, Haiyan Yu, Song Liu, Shenkui PLoS One Research Article The 636-bp-long cDNA sequence of OsCu/Zn-SOD (AK059841) was cloned from Oryza sativa var. Longjing11 via reverse transcription polymerase chain reaction (RT-PCR). The encoded protein comprised of 211 amino acids is highly homologous to Cu/Zn-SOD proteins from tuscacera rice and millet. Quantitative RT-PCR revealed that in rice, the level of OsCu/Zn-SOD gene expression was lowest in roots and was highest in petals and during the S5 leaf stage. Moreover, the expression level of OsCu/Zn-SOD gene expression decreased during the L5 leaf stage to maturity. The level of OsCu/Zn-SOD gene expression, however, was increased under saline–sodic stress and NaHCO(3) stress. Germination tests under 125, 150, and 175 mM NaCl revealed that OsCu/Zn-SOD-overexpressing lines performed better than the non-transgenic (NT) Longjing11 lines in terms of germination rate and height. Subjecting seedlings to NaHCO(3) and water stress revealed that OsCu/Zn-SOD-overexpressing lines performed better than NT in terms of SOD activity, fresh weight, root length, and height. Under simulated NaHCO(3) stress, OsCu/Zn-SOD-overexpressing lines performed better than NT in terms of survival rate (25.19% > 6.67%) and yield traits (average grain weight 20.6 > 18.15 g). This study showed that OsCu/Zn-SOD gene overexpression increases the detoxification capacity of reactive oxygen species in O. sativa and reduces salt-induced oxidative damage. We also revealed the regulatory mechanism of OsCu/Zn-SOD enzyme in saline–sodic stress resistance in O. sativa. Moreover, we provided an experimental foundation for studying the mechanism of OsCu/Zn-SOD enzymes in the chloroplast. Public Library of Science 2017-10-11 /pmc/articles/PMC5636109/ /pubmed/29020034 http://dx.doi.org/10.1371/journal.pone.0186052 Text en © 2017 Guan et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Guan, Qingjie
Liao, Xu
He, Mingliang
Li, Xiufeng
Wang, Zhenyu
Ma, Haiyan
Yu, Song
Liu, Shenkui
Tolerance analysis of chloroplast OsCu/Zn-SOD overexpressing rice under NaCl and NaHCO(3) stress
title Tolerance analysis of chloroplast OsCu/Zn-SOD overexpressing rice under NaCl and NaHCO(3) stress
title_full Tolerance analysis of chloroplast OsCu/Zn-SOD overexpressing rice under NaCl and NaHCO(3) stress
title_fullStr Tolerance analysis of chloroplast OsCu/Zn-SOD overexpressing rice under NaCl and NaHCO(3) stress
title_full_unstemmed Tolerance analysis of chloroplast OsCu/Zn-SOD overexpressing rice under NaCl and NaHCO(3) stress
title_short Tolerance analysis of chloroplast OsCu/Zn-SOD overexpressing rice under NaCl and NaHCO(3) stress
title_sort tolerance analysis of chloroplast oscu/zn-sod overexpressing rice under nacl and nahco(3) stress
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5636109/
https://www.ncbi.nlm.nih.gov/pubmed/29020034
http://dx.doi.org/10.1371/journal.pone.0186052
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