Tolerance analysis of chloroplast OsCu/Zn-SOD overexpressing rice under NaCl and NaHCO(3) stress
The 636-bp-long cDNA sequence of OsCu/Zn-SOD (AK059841) was cloned from Oryza sativa var. Longjing11 via reverse transcription polymerase chain reaction (RT-PCR). The encoded protein comprised of 211 amino acids is highly homologous to Cu/Zn-SOD proteins from tuscacera rice and millet. Quantitative...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5636109/ https://www.ncbi.nlm.nih.gov/pubmed/29020034 http://dx.doi.org/10.1371/journal.pone.0186052 |
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author | Guan, Qingjie Liao, Xu He, Mingliang Li, Xiufeng Wang, Zhenyu Ma, Haiyan Yu, Song Liu, Shenkui |
author_facet | Guan, Qingjie Liao, Xu He, Mingliang Li, Xiufeng Wang, Zhenyu Ma, Haiyan Yu, Song Liu, Shenkui |
author_sort | Guan, Qingjie |
collection | PubMed |
description | The 636-bp-long cDNA sequence of OsCu/Zn-SOD (AK059841) was cloned from Oryza sativa var. Longjing11 via reverse transcription polymerase chain reaction (RT-PCR). The encoded protein comprised of 211 amino acids is highly homologous to Cu/Zn-SOD proteins from tuscacera rice and millet. Quantitative RT-PCR revealed that in rice, the level of OsCu/Zn-SOD gene expression was lowest in roots and was highest in petals and during the S5 leaf stage. Moreover, the expression level of OsCu/Zn-SOD gene expression decreased during the L5 leaf stage to maturity. The level of OsCu/Zn-SOD gene expression, however, was increased under saline–sodic stress and NaHCO(3) stress. Germination tests under 125, 150, and 175 mM NaCl revealed that OsCu/Zn-SOD-overexpressing lines performed better than the non-transgenic (NT) Longjing11 lines in terms of germination rate and height. Subjecting seedlings to NaHCO(3) and water stress revealed that OsCu/Zn-SOD-overexpressing lines performed better than NT in terms of SOD activity, fresh weight, root length, and height. Under simulated NaHCO(3) stress, OsCu/Zn-SOD-overexpressing lines performed better than NT in terms of survival rate (25.19% > 6.67%) and yield traits (average grain weight 20.6 > 18.15 g). This study showed that OsCu/Zn-SOD gene overexpression increases the detoxification capacity of reactive oxygen species in O. sativa and reduces salt-induced oxidative damage. We also revealed the regulatory mechanism of OsCu/Zn-SOD enzyme in saline–sodic stress resistance in O. sativa. Moreover, we provided an experimental foundation for studying the mechanism of OsCu/Zn-SOD enzymes in the chloroplast. |
format | Online Article Text |
id | pubmed-5636109 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-56361092017-10-30 Tolerance analysis of chloroplast OsCu/Zn-SOD overexpressing rice under NaCl and NaHCO(3) stress Guan, Qingjie Liao, Xu He, Mingliang Li, Xiufeng Wang, Zhenyu Ma, Haiyan Yu, Song Liu, Shenkui PLoS One Research Article The 636-bp-long cDNA sequence of OsCu/Zn-SOD (AK059841) was cloned from Oryza sativa var. Longjing11 via reverse transcription polymerase chain reaction (RT-PCR). The encoded protein comprised of 211 amino acids is highly homologous to Cu/Zn-SOD proteins from tuscacera rice and millet. Quantitative RT-PCR revealed that in rice, the level of OsCu/Zn-SOD gene expression was lowest in roots and was highest in petals and during the S5 leaf stage. Moreover, the expression level of OsCu/Zn-SOD gene expression decreased during the L5 leaf stage to maturity. The level of OsCu/Zn-SOD gene expression, however, was increased under saline–sodic stress and NaHCO(3) stress. Germination tests under 125, 150, and 175 mM NaCl revealed that OsCu/Zn-SOD-overexpressing lines performed better than the non-transgenic (NT) Longjing11 lines in terms of germination rate and height. Subjecting seedlings to NaHCO(3) and water stress revealed that OsCu/Zn-SOD-overexpressing lines performed better than NT in terms of SOD activity, fresh weight, root length, and height. Under simulated NaHCO(3) stress, OsCu/Zn-SOD-overexpressing lines performed better than NT in terms of survival rate (25.19% > 6.67%) and yield traits (average grain weight 20.6 > 18.15 g). This study showed that OsCu/Zn-SOD gene overexpression increases the detoxification capacity of reactive oxygen species in O. sativa and reduces salt-induced oxidative damage. We also revealed the regulatory mechanism of OsCu/Zn-SOD enzyme in saline–sodic stress resistance in O. sativa. Moreover, we provided an experimental foundation for studying the mechanism of OsCu/Zn-SOD enzymes in the chloroplast. Public Library of Science 2017-10-11 /pmc/articles/PMC5636109/ /pubmed/29020034 http://dx.doi.org/10.1371/journal.pone.0186052 Text en © 2017 Guan et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Guan, Qingjie Liao, Xu He, Mingliang Li, Xiufeng Wang, Zhenyu Ma, Haiyan Yu, Song Liu, Shenkui Tolerance analysis of chloroplast OsCu/Zn-SOD overexpressing rice under NaCl and NaHCO(3) stress |
title | Tolerance analysis of chloroplast OsCu/Zn-SOD overexpressing rice under NaCl and NaHCO(3) stress |
title_full | Tolerance analysis of chloroplast OsCu/Zn-SOD overexpressing rice under NaCl and NaHCO(3) stress |
title_fullStr | Tolerance analysis of chloroplast OsCu/Zn-SOD overexpressing rice under NaCl and NaHCO(3) stress |
title_full_unstemmed | Tolerance analysis of chloroplast OsCu/Zn-SOD overexpressing rice under NaCl and NaHCO(3) stress |
title_short | Tolerance analysis of chloroplast OsCu/Zn-SOD overexpressing rice under NaCl and NaHCO(3) stress |
title_sort | tolerance analysis of chloroplast oscu/zn-sod overexpressing rice under nacl and nahco(3) stress |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5636109/ https://www.ncbi.nlm.nih.gov/pubmed/29020034 http://dx.doi.org/10.1371/journal.pone.0186052 |
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