Combined CRISPRi/a-Based Chemical Genetic Screens Reveal that Rigosertib Is a Microtubule-Destabilizing Agent

Chemical libraries paired with phenotypic screens can now readily identify compounds with therapeutic potential. A central limitation to exploiting these compounds, however, has been in identifying their relevant cellular targets. Here, we present a two-tiered CRISPR-mediated chemical-genetic strate...

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Detalles Bibliográficos
Autores principales: Jost, Marco, Chen, Yuwen, Gilbert, Luke A., Horlbeck, Max A., Krenning, Lenno, Menchon, Grégory, Rai, Ankit, Cho, Min Y., Stern, Jacob J., Prota, Andrea E., Kampmann, Martin, Akhmanova, Anna, Steinmetz, Michel O., Tanenbaum, Marvin E., Weissman, Jonathan S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5640507/
https://www.ncbi.nlm.nih.gov/pubmed/28985505
http://dx.doi.org/10.1016/j.molcel.2017.09.012

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5640507/
https://www.ncbi.nlm.nih.gov/pubmed/28985505
http://dx.doi.org/10.1016/j.molcel.2017.09.012

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