Cargando…

Yeast knockout library allows for efficient testing of genomic mutations for cell-free protein synthesis

Cell-free protein synthesis (CFPS) systems from crude lysates have benefitted from modifications to their enzyme composition. For example, functionally deleting enzymes in the source strain that are deleterious to CFPS can improve protein synthesis yields. However, making such modifications can take...

Descripción completa

Detalles Bibliográficos
Autores principales: Schoborg, Jennifer A., Clark, Lauren G., Choudhury, Alaksh, Hodgman, C. Eric, Jewett, Michael C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5640588/
https://www.ncbi.nlm.nih.gov/pubmed/29062921
http://dx.doi.org/10.1016/j.synbio.2016.02.004