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Sequence homolog-based molecular engineering for shifting the enzymatic pH optimum

Cell-free synthetic biology system organizes multiple enzymes (parts) from different sources to implement unnatural catalytic functions. Highly adaption between the catalytic parts is crucial for building up efficient artificial biosynthetic systems. Protein engineering is a powerful technology to t...

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Detalles Bibliográficos
Autores principales: Ma, Fuqiang, Xie, Yuan, Luo, Manjie, Wang, Shuhao, Hu, You, Liu, Yukun, Feng, Yan, Yang, Guang-Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5640797/
https://www.ncbi.nlm.nih.gov/pubmed/29062943
http://dx.doi.org/10.1016/j.synbio.2016.09.001