Phage-assisted continuous evolution of proteases with altered substrate specificity

Here we perform phage-assisted continuous evolution (PACE) of TEV protease, which canonically cleaves ENLYFQS, to cleave a very different target sequence, HPLVGHM, that is present in human IL-23. A protease emerging from ∼2500 generations of PACE contains 20 non-silent mutations, cleaves human IL-23...

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Detalles Bibliográficos
Autores principales: Packer, Michael S., Rees, Holly A., Liu, David R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5643515/
https://www.ncbi.nlm.nih.gov/pubmed/29038472
http://dx.doi.org/10.1038/s41467-017-01055-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5643515/
https://www.ncbi.nlm.nih.gov/pubmed/29038472
http://dx.doi.org/10.1038/s41467-017-01055-9

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