Development and Evaluation of a Novel Taqman Real-Time PCR Assay for Rapid Detection of Mycoplasma bovis: Comparison of Assay Performance with a Conventional PCR Assay and Another Taqman Real-Time PCR Assay ‡

The objective of this study was to develop and validate a Taqman real-time PCR assay for the detection of Mycoplasma bovis (M. bovis). Unique primers targeting the highly conserved house-keeping gene (uvrC) were designed and the probe sequence was derived from a previously published microarray study...

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Detalles Bibliográficos
Autores principales: Naikare, Hemant, Bruno, Daniela, Mahapatra, Debabrata, Reinisch, Alesia, Raleigh, Russell, Sprowls, Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5644610/
https://www.ncbi.nlm.nih.gov/pubmed/29061929
http://dx.doi.org/10.3390/vetsci2010032

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5644610/
https://www.ncbi.nlm.nih.gov/pubmed/29061929
http://dx.doi.org/10.3390/vetsci2010032

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