Multiple exo-glycosidases in human serum as detected with the substrate DNP-α-GalNAc. I. A new assay for lysosomal α-N-acetylgalactosaminidase

This paper presents a new assay to determine the activity of the lysosomal enzyme α-N-acetylgalactosaminidase (Naga, EC 3.2.1.49) in human serum. It is based on the use of a new chromogenic substrate, DNP-α-GalNAc (2,4-dinitrophenyl-N-acetyl-α-D-galactosaminide) and is performed at pH 4.3 and 37 °C....

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Detalles Bibliográficos
Autores principales: Albracht, Simon P.J., Allon, Erik, van Pelt, Johannes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Regular Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5645117/
https://www.ncbi.nlm.nih.gov/pubmed/29062717
http://dx.doi.org/10.1016/j.bbacli.2017.10.001

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5645117/
https://www.ncbi.nlm.nih.gov/pubmed/29062717
http://dx.doi.org/10.1016/j.bbacli.2017.10.001

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