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Simultaneous Quantification of Free Cholesterol, Cholesteryl Esters, and Triglycerides without Ester Hydrolysis by UHPLC Separation and In-Source Collision Induced Dissociation Coupled MS/MS

We demonstrate the application of in-source nitrogen collision-induced dissociation (CID) that eliminates the need for ester hydrolysis before simultaneous analysis of esterified cholesterol (EC) and triglycerides (TG) along with free cholesterol (FC) from human serum, using normal phase liquid chro...

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Detalles Bibliográficos
Autores principales: Gardner, Michael S., McWilliams, Lisa G., Jones, Jeffrey I., Kuklenyik, Zsuzsanna, Pirkle, James L., Barr, John R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5645443/
https://www.ncbi.nlm.nih.gov/pubmed/28801822
http://dx.doi.org/10.1007/s13361-017-1756-2