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CRISPR/Cas-mediated knock-in via non-homologous end-joining in the crustacean Daphnia magna

The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated system (Cas) is widely used for mediating the knock-in of foreign DNA into the genomes of various organisms. Here, we report a process of CRISPR/Cas-mediated knock-in via non-homologous end joining by the direct...

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Detalles Bibliográficos
Autores principales: Kumagai, Hitoshi, Nakanishi, Takashi, Matsuura, Tomoaki, Kato, Yasuhiko, Watanabe, Hajime
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5646780/
https://www.ncbi.nlm.nih.gov/pubmed/29045453
http://dx.doi.org/10.1371/journal.pone.0186112