Cryo-EM Visualization of Lipid and Polymer-Stabilized Perfluorocarbon Gas Nanobubbles - A Step Towards Nanobubble Mediated Drug Delivery
Gas microbubbles stabilized with lipids, surfactants, proteins and/or polymers are widely used clinically as ultrasound contrast agents. Because of their large 1–10 µm size, applications of microbubbles are confined to the blood vessels. Accordingly, there is much interest in generating nanoscale ec...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5647366/ https://www.ncbi.nlm.nih.gov/pubmed/29044154 http://dx.doi.org/10.1038/s41598-017-13741-1 |
_version_ | 1783272230722994176 |
---|---|
author | Hernandez, Christopher Gulati, Sahil Fioravanti, Gabriella Stewart, Phoebe L. Exner, Agata A. |
author_facet | Hernandez, Christopher Gulati, Sahil Fioravanti, Gabriella Stewart, Phoebe L. Exner, Agata A. |
author_sort | Hernandez, Christopher |
collection | PubMed |
description | Gas microbubbles stabilized with lipids, surfactants, proteins and/or polymers are widely used clinically as ultrasound contrast agents. Because of their large 1–10 µm size, applications of microbubbles are confined to the blood vessels. Accordingly, there is much interest in generating nanoscale echogenic bubbles (nanobubbles), which can enable new uses of ultrasound contrast agents in molecular imaging and drug delivery, particularly for cancer applications. While the interactions of microbubbles with ultrasound have been widely investigated, little is known about the activity of nanobubbles under ultrasound exposure. In this work, we demonstrate that cryo-electron microscopy (cryo-EM) can be used to image nanoscale lipid and polymer-stabilized perfluorocarbon gas bubbles before and after their destruction with high intensity ultrasound. In addition, cryo-EM can be used to observe electron-beam induced dissipation of nanobubble encapsulated perfluorocarbon gas. |
format | Online Article Text |
id | pubmed-5647366 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-56473662017-10-26 Cryo-EM Visualization of Lipid and Polymer-Stabilized Perfluorocarbon Gas Nanobubbles - A Step Towards Nanobubble Mediated Drug Delivery Hernandez, Christopher Gulati, Sahil Fioravanti, Gabriella Stewart, Phoebe L. Exner, Agata A. Sci Rep Article Gas microbubbles stabilized with lipids, surfactants, proteins and/or polymers are widely used clinically as ultrasound contrast agents. Because of their large 1–10 µm size, applications of microbubbles are confined to the blood vessels. Accordingly, there is much interest in generating nanoscale echogenic bubbles (nanobubbles), which can enable new uses of ultrasound contrast agents in molecular imaging and drug delivery, particularly for cancer applications. While the interactions of microbubbles with ultrasound have been widely investigated, little is known about the activity of nanobubbles under ultrasound exposure. In this work, we demonstrate that cryo-electron microscopy (cryo-EM) can be used to image nanoscale lipid and polymer-stabilized perfluorocarbon gas bubbles before and after their destruction with high intensity ultrasound. In addition, cryo-EM can be used to observe electron-beam induced dissipation of nanobubble encapsulated perfluorocarbon gas. Nature Publishing Group UK 2017-10-18 /pmc/articles/PMC5647366/ /pubmed/29044154 http://dx.doi.org/10.1038/s41598-017-13741-1 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Hernandez, Christopher Gulati, Sahil Fioravanti, Gabriella Stewart, Phoebe L. Exner, Agata A. Cryo-EM Visualization of Lipid and Polymer-Stabilized Perfluorocarbon Gas Nanobubbles - A Step Towards Nanobubble Mediated Drug Delivery |
title | Cryo-EM Visualization of Lipid and Polymer-Stabilized Perfluorocarbon Gas Nanobubbles - A Step Towards Nanobubble Mediated Drug Delivery |
title_full | Cryo-EM Visualization of Lipid and Polymer-Stabilized Perfluorocarbon Gas Nanobubbles - A Step Towards Nanobubble Mediated Drug Delivery |
title_fullStr | Cryo-EM Visualization of Lipid and Polymer-Stabilized Perfluorocarbon Gas Nanobubbles - A Step Towards Nanobubble Mediated Drug Delivery |
title_full_unstemmed | Cryo-EM Visualization of Lipid and Polymer-Stabilized Perfluorocarbon Gas Nanobubbles - A Step Towards Nanobubble Mediated Drug Delivery |
title_short | Cryo-EM Visualization of Lipid and Polymer-Stabilized Perfluorocarbon Gas Nanobubbles - A Step Towards Nanobubble Mediated Drug Delivery |
title_sort | cryo-em visualization of lipid and polymer-stabilized perfluorocarbon gas nanobubbles - a step towards nanobubble mediated drug delivery |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5647366/ https://www.ncbi.nlm.nih.gov/pubmed/29044154 http://dx.doi.org/10.1038/s41598-017-13741-1 |
work_keys_str_mv | AT hernandezchristopher cryoemvisualizationoflipidandpolymerstabilizedperfluorocarbongasnanobubblesasteptowardsnanobubblemediateddrugdelivery AT gulatisahil cryoemvisualizationoflipidandpolymerstabilizedperfluorocarbongasnanobubblesasteptowardsnanobubblemediateddrugdelivery AT fioravantigabriella cryoemvisualizationoflipidandpolymerstabilizedperfluorocarbongasnanobubblesasteptowardsnanobubblemediateddrugdelivery AT stewartphoebel cryoemvisualizationoflipidandpolymerstabilizedperfluorocarbongasnanobubblesasteptowardsnanobubblemediateddrugdelivery AT exneragataa cryoemvisualizationoflipidandpolymerstabilizedperfluorocarbongasnanobubblesasteptowardsnanobubblemediateddrugdelivery |