Cryo-EM Visualization of Lipid and Polymer-Stabilized Perfluorocarbon Gas Nanobubbles - A Step Towards Nanobubble Mediated Drug Delivery

Gas microbubbles stabilized with lipids, surfactants, proteins and/or polymers are widely used clinically as ultrasound contrast agents. Because of their large 1–10 µm size, applications of microbubbles are confined to the blood vessels. Accordingly, there is much interest in generating nanoscale ec...

Descripción completa

Detalles Bibliográficos
Autores principales: Hernandez, Christopher, Gulati, Sahil, Fioravanti, Gabriella, Stewart, Phoebe L., Exner, Agata A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5647366/
https://www.ncbi.nlm.nih.gov/pubmed/29044154
http://dx.doi.org/10.1038/s41598-017-13741-1
_version_ 1783272230722994176
author Hernandez, Christopher
Gulati, Sahil
Fioravanti, Gabriella
Stewart, Phoebe L.
Exner, Agata A.
author_facet Hernandez, Christopher
Gulati, Sahil
Fioravanti, Gabriella
Stewart, Phoebe L.
Exner, Agata A.
author_sort Hernandez, Christopher
collection PubMed
description Gas microbubbles stabilized with lipids, surfactants, proteins and/or polymers are widely used clinically as ultrasound contrast agents. Because of their large 1–10 µm size, applications of microbubbles are confined to the blood vessels. Accordingly, there is much interest in generating nanoscale echogenic bubbles (nanobubbles), which can enable new uses of ultrasound contrast agents in molecular imaging and drug delivery, particularly for cancer applications. While the interactions of microbubbles with ultrasound have been widely investigated, little is known about the activity of nanobubbles under ultrasound exposure. In this work, we demonstrate that cryo-electron microscopy (cryo-EM) can be used to image nanoscale lipid and polymer-stabilized perfluorocarbon gas bubbles before and after their destruction with high intensity ultrasound. In addition, cryo-EM can be used to observe electron-beam induced dissipation of nanobubble encapsulated perfluorocarbon gas.
format Online
Article
Text
id pubmed-5647366
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-56473662017-10-26 Cryo-EM Visualization of Lipid and Polymer-Stabilized Perfluorocarbon Gas Nanobubbles - A Step Towards Nanobubble Mediated Drug Delivery Hernandez, Christopher Gulati, Sahil Fioravanti, Gabriella Stewart, Phoebe L. Exner, Agata A. Sci Rep Article Gas microbubbles stabilized with lipids, surfactants, proteins and/or polymers are widely used clinically as ultrasound contrast agents. Because of their large 1–10 µm size, applications of microbubbles are confined to the blood vessels. Accordingly, there is much interest in generating nanoscale echogenic bubbles (nanobubbles), which can enable new uses of ultrasound contrast agents in molecular imaging and drug delivery, particularly for cancer applications. While the interactions of microbubbles with ultrasound have been widely investigated, little is known about the activity of nanobubbles under ultrasound exposure. In this work, we demonstrate that cryo-electron microscopy (cryo-EM) can be used to image nanoscale lipid and polymer-stabilized perfluorocarbon gas bubbles before and after their destruction with high intensity ultrasound. In addition, cryo-EM can be used to observe electron-beam induced dissipation of nanobubble encapsulated perfluorocarbon gas. Nature Publishing Group UK 2017-10-18 /pmc/articles/PMC5647366/ /pubmed/29044154 http://dx.doi.org/10.1038/s41598-017-13741-1 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Hernandez, Christopher
Gulati, Sahil
Fioravanti, Gabriella
Stewart, Phoebe L.
Exner, Agata A.
Cryo-EM Visualization of Lipid and Polymer-Stabilized Perfluorocarbon Gas Nanobubbles - A Step Towards Nanobubble Mediated Drug Delivery
title Cryo-EM Visualization of Lipid and Polymer-Stabilized Perfluorocarbon Gas Nanobubbles - A Step Towards Nanobubble Mediated Drug Delivery
title_full Cryo-EM Visualization of Lipid and Polymer-Stabilized Perfluorocarbon Gas Nanobubbles - A Step Towards Nanobubble Mediated Drug Delivery
title_fullStr Cryo-EM Visualization of Lipid and Polymer-Stabilized Perfluorocarbon Gas Nanobubbles - A Step Towards Nanobubble Mediated Drug Delivery
title_full_unstemmed Cryo-EM Visualization of Lipid and Polymer-Stabilized Perfluorocarbon Gas Nanobubbles - A Step Towards Nanobubble Mediated Drug Delivery
title_short Cryo-EM Visualization of Lipid and Polymer-Stabilized Perfluorocarbon Gas Nanobubbles - A Step Towards Nanobubble Mediated Drug Delivery
title_sort cryo-em visualization of lipid and polymer-stabilized perfluorocarbon gas nanobubbles - a step towards nanobubble mediated drug delivery
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5647366/
https://www.ncbi.nlm.nih.gov/pubmed/29044154
http://dx.doi.org/10.1038/s41598-017-13741-1
work_keys_str_mv AT hernandezchristopher cryoemvisualizationoflipidandpolymerstabilizedperfluorocarbongasnanobubblesasteptowardsnanobubblemediateddrugdelivery
AT gulatisahil cryoemvisualizationoflipidandpolymerstabilizedperfluorocarbongasnanobubblesasteptowardsnanobubblemediateddrugdelivery
AT fioravantigabriella cryoemvisualizationoflipidandpolymerstabilizedperfluorocarbongasnanobubblesasteptowardsnanobubblemediateddrugdelivery
AT stewartphoebel cryoemvisualizationoflipidandpolymerstabilizedperfluorocarbongasnanobubblesasteptowardsnanobubblemediateddrugdelivery
AT exneragataa cryoemvisualizationoflipidandpolymerstabilizedperfluorocarbongasnanobubblesasteptowardsnanobubblemediateddrugdelivery