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Role of blocking ADAM10 hydrolysis site on N-cadherin by single-chain antibody in ventricular remodeling

The present study aimed to investigate the roles of the hydrolytic process of N-cadherin by A disintegrin and metalloproteases 10 (ADAM10) in sustaining myocardial structure and integrity, and discuss the mechanisms of ventricular remodeling in dilated cardiomyopathy (DCM). Single chain variable fra...

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Autores principales: Li, Xiaoou, Huang, Wei, He, Bing, Zhou, Lirong, Huang, Xiaogang, Yao, Baozhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5647691/
https://www.ncbi.nlm.nih.gov/pubmed/29067106
http://dx.doi.org/10.3892/etm.2017.5057
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author Li, Xiaoou
Huang, Wei
He, Bing
Zhou, Lirong
Huang, Xiaogang
Yao, Baozhen
author_facet Li, Xiaoou
Huang, Wei
He, Bing
Zhou, Lirong
Huang, Xiaogang
Yao, Baozhen
author_sort Li, Xiaoou
collection PubMed
description The present study aimed to investigate the roles of the hydrolytic process of N-cadherin by A disintegrin and metalloproteases 10 (ADAM10) in sustaining myocardial structure and integrity, and discuss the mechanisms of ventricular remodeling in dilated cardiomyopathy (DCM). Single chain variable fragment antibody (ScFv) with the ability to specifically block the ADAM10 hydrolysis site of N-cadherin was designed and constructed. Western blot analysis and flow cytometry were used to detect the expression of N-cadherin and its C-terminal fragment 1 (CTF1) on cardiomyocytes, and cells were also subjected to a cell adhesion assay. Furthermore, in a rat model of dilated cardiomyopathy (DCM), the effects of intracardiac injection of the recombinant adenovirus on cardiac structure and contractile function were observed by hematoxylin and eosin staining and color Doppler echocardiography. The recombinant ScFv-expressing adenoviral plasmid with the ability to block the ADAM10 hydrolysis site on N-cadherin was successfully constructed and efficiently transfected into H9C2 cells. After transfection, N-cadherin protein expression was significantly increased, CTF1 protein was significantly decreased and the adhesion capability of myocardial cells was significantly improved. In the in vivo experiment, N-cadherin expression was significantly increased in the treatment group compared with that in the model group, and the structure and function of the heart were significantly improved. In conclusion, blocking of the ADAM10 hydrolysis site on N-cadherin by ScFv increased N-cadherin expression and improved ventricular remodeling. The present study provided experimental evidence for a novel approach for the treatment and prevention of DCM.
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spelling pubmed-56476912017-10-24 Role of blocking ADAM10 hydrolysis site on N-cadherin by single-chain antibody in ventricular remodeling Li, Xiaoou Huang, Wei He, Bing Zhou, Lirong Huang, Xiaogang Yao, Baozhen Exp Ther Med Articles The present study aimed to investigate the roles of the hydrolytic process of N-cadherin by A disintegrin and metalloproteases 10 (ADAM10) in sustaining myocardial structure and integrity, and discuss the mechanisms of ventricular remodeling in dilated cardiomyopathy (DCM). Single chain variable fragment antibody (ScFv) with the ability to specifically block the ADAM10 hydrolysis site of N-cadherin was designed and constructed. Western blot analysis and flow cytometry were used to detect the expression of N-cadherin and its C-terminal fragment 1 (CTF1) on cardiomyocytes, and cells were also subjected to a cell adhesion assay. Furthermore, in a rat model of dilated cardiomyopathy (DCM), the effects of intracardiac injection of the recombinant adenovirus on cardiac structure and contractile function were observed by hematoxylin and eosin staining and color Doppler echocardiography. The recombinant ScFv-expressing adenoviral plasmid with the ability to block the ADAM10 hydrolysis site on N-cadherin was successfully constructed and efficiently transfected into H9C2 cells. After transfection, N-cadherin protein expression was significantly increased, CTF1 protein was significantly decreased and the adhesion capability of myocardial cells was significantly improved. In the in vivo experiment, N-cadherin expression was significantly increased in the treatment group compared with that in the model group, and the structure and function of the heart were significantly improved. In conclusion, blocking of the ADAM10 hydrolysis site on N-cadherin by ScFv increased N-cadherin expression and improved ventricular remodeling. The present study provided experimental evidence for a novel approach for the treatment and prevention of DCM. D.A. Spandidos 2017-11 2017-08-28 /pmc/articles/PMC5647691/ /pubmed/29067106 http://dx.doi.org/10.3892/etm.2017.5057 Text en Copyright: © Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Li, Xiaoou
Huang, Wei
He, Bing
Zhou, Lirong
Huang, Xiaogang
Yao, Baozhen
Role of blocking ADAM10 hydrolysis site on N-cadherin by single-chain antibody in ventricular remodeling
title Role of blocking ADAM10 hydrolysis site on N-cadherin by single-chain antibody in ventricular remodeling
title_full Role of blocking ADAM10 hydrolysis site on N-cadherin by single-chain antibody in ventricular remodeling
title_fullStr Role of blocking ADAM10 hydrolysis site on N-cadherin by single-chain antibody in ventricular remodeling
title_full_unstemmed Role of blocking ADAM10 hydrolysis site on N-cadherin by single-chain antibody in ventricular remodeling
title_short Role of blocking ADAM10 hydrolysis site on N-cadherin by single-chain antibody in ventricular remodeling
title_sort role of blocking adam10 hydrolysis site on n-cadherin by single-chain antibody in ventricular remodeling
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5647691/
https://www.ncbi.nlm.nih.gov/pubmed/29067106
http://dx.doi.org/10.3892/etm.2017.5057
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